Contact an Account ManagerHydrophobic interaction chromatography in the detection of hemoglobinopathies
Summary
Type: HPLC
Application #: 108490
Catalog#: 720013.40 - MN HPLC Column, Nucleosil C8, 100A, 5um, 4x250mm
Detection: UV, 215nm
Conditions: 0.155M NaCl pH 2.7 - methanol - acetonitrile - 0.077M NaCl pH 2.7, gradient from 1:33:26:41 to 10:23:42:25
flow rate 1ml/min
temperature 48°C; detection: UV, 215nm
Authors: Parviainen MT
Source: J Liquid Chromatogr 10 (1987) 2951 - 2963
Details
Legend: Fig. 1. Separation of different globin chains in red blood cell lysates by
hydrophobic interaction chromatography, A) elution of Hb chains from a normal
adult HbA homozygote, B) Hb chains from a new